JVI Accepts, published online ahead of print on 6 June 2007
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.
EFFICIENT IN VITRO AMPLIFICATION OF CHRONIC WASTING DISEASE PrPRES
Department of Microbiology, Immunology, and
Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado
State University; Department of Biochemistry, Dartmouth Medical School,
Department of Molecular Biology and Genetics, University of Kentucky
* To whom correspondence should be addressed. Email: edward.hoover@colostate.edu .
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Abstract |
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Chronic wasting disease (CWD) of cervids is associated with conversion of the normal cervid prion protein, PrPC, to a protease-resistant conformer, PrPCWD. Here we report use of both non-denaturing amplification and protein-misfolding cyclic amplification (PMCA) to amplify PrPCWD in vitro. Normal brain from deer, transgenic mice [Tg(cerPrP)1536] expressing cervid PrPC, and ferrets supported amplification. PMCA using Tg(cerPrP)1536 normal brain as PrPC substrate produced amplification of >6.5 x 109-fold after six rounds. Highly efficient in vitro amplification of PrPCWD is a significant step toward detection of PrPCWD in body fluids or excreta of CWD-susceptible species.
http://jvi.asm.org/cgi/content/abstract/JVI.00635-07v1?papetoc